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Welcome

New funding

11/11/2017

 
With 2 successful research grants in a week, we are getting ready to look into the evolution of ascetosporean parasites (Rhizaria), currently a black box on the map of microbial eukaryote parasites.

Warmest thanks to our funding agencies VR and Formas for their support.

Postdoc position available: comparative genomics of poorly known protist parasites

9/8/2017

 
Apply online

Brief description:
 Study the evolution and specific adaptations to parasitism in Ascetosporea, an emerging threat in marine environments that has remained beyond reach of modern molecular tools. Ascetosporea is a collection of poorly characterized microbial eukaryote parasites of marine invertebrates. It is best-known for including deadly pathogens of oysters and mussels, costing millions of dollars to the growing aquaculture industry. To date, however, Ascetosporea is absent from models describing the evolution of parasites because comparative genomic data is lacking. This is due to multiple factors, including minute cell size, obligate intracellular lifestyle, lack of suitable host cell lines, and techniques for in vitro culture. Here, we propose to overcome these challenges by using micromanipulation and single-cell genomic/transcriptomic methods. We will sequence de novo the genome and transcriptome of several uncultured parasites encompassing the diversity of Ascetosporea, as well as their closest free-living outgroups. With these data, we will broadly look at the evolution of parasitism in Ascetosporea, i.e. identify pre-parasitic conditions, ancestral parasitic innovations, and lineage-specific adaptations using comparative genomics.

​Full description here:
scilife_postdoctoral_fellow.pdf
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Master project available to work on plastid metagenomics

7/6/2017

 
project_plastidmetagenome.pdf
File Size: 378 kb
File Type: pdf
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New lab members

18/4/2017

 
Mahwash and Vasily recently joined the lab, a warm (in the still freezing Uppsala) welcome to them!

PhD position available, deadline Oct 31

10/10/2016

 
Microbial eukaryote parasites are very diverse and widespread across the eukaryotic tree of life, but our understanding of these important organisms is mostly based on a relatively small number of species in pathogenous groups (such as Apicomplexa or Microsporidia). Other groups, composed partly or exclusively of parasites, have thus far eluded in depth investigation. Ascetosporea is one such group of poorly characterized parasites belonging to Rhizaria, itself the least studied of the major divisions of eukaryotes. The lineage diversity and host range of ascetosporean parasites have been recently found to be much larger than anticipated, among which are important pathogens of molluscs costing millions of dollars to the aquaculture industry. In spite of this, our genomic understanding of the evolution of Ascetosporea is extremely limited, and no genomes are currently available.

This project aims to de novo sequence, assemble, and annotate several genomes of Ascetosporea and related organisms. Material will be provided by collaborators (mainly in the UK) and sequence in-house. This newly generated data will then be used to establish an evolutionary framework for Ascetosporea using a combination of phylogenetics and genomic analyses. Based on the outcome of this first part, we will broadly look at the evolution of parasitism in Ascetosporea, i.e. identify pre-parasitic conditions, ancestral parasitic innovations, and lineage-specific adaptations using comparative genomics. This project will shed light on a large group of parasites for which we lack even the most fundamental knowledge about their biology. As part of a more general aim to sequence uncultured micro-eukaryotes, the student will also be involved in developing single-cell genome sequencing approaches.

THIS POSITION HAS NOW BEEN FILLED

Mitochondrial Evolution: Going, Going, Gone. Curr. Biol. 26(10):R410-412.

24/5/2016

 
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Postdoc/Researcher position available.

20/2/2016

 
This position is available to work with me on protist phylogenomics, in the Systematic Biology Department at Uppsala University (Sweden). More info here.

​THIS POSITION HAS NOW BEEN FILLED

Untangling the early diversification of eukaryotes: a phylogenomic study of the evolutionary origins of Centrohelida, Haptophyta and Cryptista. Proc. R. Soc. B. 283: 20152802.

27/1/2016

 
Assembling the global eukaryotic tree of life has long been a major effort of Biology. In recent years, pushed by the new availability of genome-scale data for microbial eukaryotes, it has become possible to revisit many evolution- ary enigmas. However, some of the most ancient nodes, which are essential for inferring a stable tree, have remained highly controversial. Among other reasons, the lack of adequate genomic datasets for key taxa has prevented the robust reconstruction of early diversification events. In this context, the cen- trohelid heliozoans are particularly relevant for reconstructing the tree of eukaryotes because they represent one of the last substantial groups that was missing large and diverse genomic data. Here, we filled this gap by sequencing high-quality transcriptomes for four centrohelid lineages, each correspond- ing to a different family. Combining these new data with a broad eukaryotic sampling, we produced a gene-rich taxon-rich phylogenomic dataset that enabled us to refine the structure of the tree. Specifically, we show that (i) cen- trohelids relate to haptophytes, confirming Haptista; (ii) Haptista relates to SAR; (iii) Cryptista share strong affinity with Archaeplastida; and (iv) Haptista þ SAR is sister to Cryptista þ Archaeplastida. The implications of this topology are discussed in the broader context of plastid evolution. 
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The tree of life gets a makeover

13/10/2015

 
A great pop ScienceNews article by Susan Milius on shaking the branches of the tree of life, featuring a short but sweet quotation from yours truly ;-) Article here

Go Protists Go...

27/4/2015

 
A great essay on why protists are cool, by Cyrus Martin in Current Biology:
Biology's dark matter. 2015. 25: R301–R327. Current Biology.
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    • Fabien (asst prof)
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