We are very excited for the publication of our habitat transition analysis to now be published in its final format in Nature Ecology and Evolution. The paper, which represent a major chunk of Mahwash's PhD, is available in full OA here. We used our approach of long-read metabarcoding (nearly 17K eukaryotic OTUs spanning the 18S-28S are presented) to reconstruct a phylogeny of eukaryotic diversity, and map onto it habitat transitions from marine to non-marine environments and vice versa (crossing the salt barrier). We also did ancestral state reconstruction to infer the habitat origin of the major eukaryotic groups. We show that species have successfully crossed the salt barrier more often that classically assumed, which likely was an important contributor to the diversification of life into what we can observe today.